Primary Identifier | MGI:5750303 | Allele Type | Targeted |
Attribute String | Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A floxed pgk-neo-STOP cassette, Loxl2 cDNA, IRES and EGFP were inserted via homologous recombination. Cre mediated recombination removed the pgk-neo-STOP cassette. Fluorescence imaging, semi-quantitative PCR, and Western blot analysis of whole-cell extracts and conditioned medium confirmed targeted overexpression in newborn homozygous mutant mice and derived MEFs. |