| Primary Identifier | MGI:5752968 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ntn1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector contains a FRT-flanked Neo selection cassette and a loxP site was inserted downstream of the first protein coding exon of the targeted gene, and another loxP site was inserted upstream of the first protein coding exon. Flp-mediated recombination removed the FRT-flanked neo cassette. Cre-mediated recombination removed the first protein coding exon. Western blot and immunohistochemical analysis of spinal cord confirmed the absence of protein expression. |
