| Primary Identifier | MGI:5752588 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mcam |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 2. An additional loxP site was inserted downstream of exon 7. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exons 2 through 7. Western blot analysis confirmed the absence of protein expression in lung microvascular endothelial cells. |
