Primary Identifier | MGI:5754793 | Allele Type | Targeted |
Attribute String | Reporter | Gene | Sema3f |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
description | ES cells = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. |
molecularNote | A lacZ cassette was inserted into exon 3. A floxed neomycin resistance cassette was inserted downstream of exon 3. This allele was used to generate Sema3ftm1Atai. |