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Allele : Col1a1<tm20(tetO-GFP,-cas9*D10A)Slowe> collagen, type I, alpha 1; targeted mutation 20, Scott Lowe
Primary Identifier  MGI:5755843 Allele Type  Targeted
Attribute String  Endonuclease, Inducible, Reporter Gene  Col1a1
Transmission  Germline Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
description  The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae (J:159351). This allele was generated with Col1a1tm13(neo/hygro*)Jae.
molecularNote  RMCE on KH2 cells inserted into the 3'UTR a RNU6 promoter driving expression of a paired guide RNAs targeting murine Apc 12 bp immediately upstream of the NGG protoscpacer-adjacent motif (PAM) sequence followed by a tetracycline response element promoter driving inducible expression of a GFP report gene, IRES sequence, human codon optimized CRISPR-associated endonuclease nickase variant cas9D10A (Cas9n; derived from Streptococcus pyogenes CRISPR associated protein 9 [SpCas9]) and polyadenylation sequence.
  • mutations:
  • Insertion
  • synonyms:
  • Apc<n>,
  • Col1a1<tm20(RNU6-gRNA:Apc,tetO-GFP,-cas9*D10A)Slowe>,
  • Apc<n>,
  • Col1a1<tm20(RNU6-gRNA:Apc,tetO-GFP,-cas9*D10A)Slowe>
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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