| Primary Identifier | MGI:5766345 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Piezo1 |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The L1L2_Bact_P cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. The FRT-lacZ-loxP-neo-FRT-loxP cassette is introduced into intron 19 followed by a loxP site placed in intron 23. Flp-mediated recombination excised the lacZ and neomycin features, leaving exons 20-23 flanked by loxP sites. |
