| Primary Identifier | MGI:5767122 | Allele Type | Targeted |
| Attribute String | Conditional ready, Hypomorph | Gene | Gbe1 |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The p.Y329S mutation (TAC to TCC change) was knocked into exon 7 and FRT sites were introduced upstream and downstream of exon 7 via homologous recombination. The loxP-flanked PGK-neomycin cassette inserted downstream of exon 7 but before the FRT site was removed via cre-mediated recombination. Western blot analysis indicates a significant reduction of protein in muscle. |
