| Primary Identifier | MGI:5776276 | Allele Type | Endonuclease-mediated |
| Attribute String | Conditional ready | Gene | Crppa |
| Strain of Origin | C57BL/6NCrl | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A conditional allele in which the second of the gene's 10 exons is flanked by loxP sites was generated using a variation of CRISPR/Ca9 technology employing paired Cas9 nickase (Cas9n) RNA with associated sgRNAs and a single DNA template containing loxP sites flanking exon 2. Cre recombinase treatment of genomic DNA from an F1 pup inheriting the PCR-verified mutation demonstrated correct excision of exon 2. |
