Primary Identifier | MGI:5776399 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Sprr3 |
Transmission | Germline | Strain of Origin | 129S/SvEv or C57BL/6J |
Is Recombinase | false | Is Wild Type | false |
description | The targeting vector was introduced into "a hybrid 129SvEv and C57Bl/6J (129/C57) ES cell line." (J:230470) |
molecularNote | The gene was replaced with an FRT- and loxP-flanked neomycin resistance cassette. Flp-mediated recmbination removed the selection cassette. Western blot analysis confirmed the absence of protein expression in the forestomach. |