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Allele : Col1a1<tm1(tetO-cas9)Sho> collagen, type I, alpha 1; targeted mutation 1, Stuart Orkin
Primary Identifier  MGI:5781096 Allele Type  Targeted
Attribute String  Endonuclease, Inducible Gene  Col1a1
Transmission  Germline Strain of Origin  (C57BL/6 x 129S4/SvJae)F1
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
description  The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae. This allele was generated with Col1a1tm13(neo/hygro*)Jae.
molecularNote  KH2 embryonic stem cells (C57BL/6 x 129S4Sv/Jae), carrying the Gt(ROSA)26Sor allele were re-targeted to insert an FRT site-flanked PGK-Neo cassette and a promoterless hygromycin resistance cassette downstream of the Col1a1 locus. Correctly re-targeted ES cells were selected and the FRT site-flanked PGK-Neo downstream of the Col1a1 locus was replaced by electroporating both a "flip-in plasmid" containing a PGK promoter, ATG start codon, FRT site, a splice acceptor-double polyA sequence (SA-2xpA), tetO (tetracycline responsive element) with a CMV minimal promoter, and SV40 polyA signal, upstream of the hCas9 (human codon optimized Cas9 nuclease) sequence, and a FLPe recombinase expressing vector to facilitate tetO-hCAS9 recombination into the 3' UTR of the Col1a1 locus.
  • mutations:
  • Insertion
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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3 Carried By

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