Primary Identifier | MGI:5781149 | Allele Type | Targeted |
Attribute String | Hypomorph | Gene | Dmd |
Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting construct inserted downstream of exon 2 a loxP site, a duplicate copy of exon 2 and an FRT-flanked neomycin resistance cassette with a 3' loxP site. mRNA analysis confirms the inclusion of a duplicated exon 2 in muscle. Immunofluorescence and immunoblot analysis indicate that expression is essentially absent, although some muscle shows very low-level trace expression. |