Primary Identifier | MGI:5784741 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Gskip |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of exon 2. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 3. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exon 2. Western blot analysis confirmed the absence of protein expression in the heart, lungs, brain and kidney at P0. |