| Primary Identifier | MGI:5787946 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rasal3 |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 6. An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 9. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exons 6 through 9. This deletion results in a frameshift and premature termination at amino acid 306. Western blot analysis confirmed the absence of protein expression in thymocytes and spleenocytes. |
