| Primary Identifier | MGI:5792821 | Allele Type | Targeted |
| Attribute String | No functional change, Recombinase | Gene | Slc17a8 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The targeting vector containes, from 5' to 3', a partial Slc17a8 sequence spanning intron 11, an frt3 site within Slc17a8 intron 11, a partial Slc17a8 exon 12 sequence up to and including the endogenous stop codon, an IRES2 sequence, a Cre recombinase, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 late polyA signal, and an AttP site. Chimeric mice were bred to PhiC31-expressing mice to remove the AttB/AttP-flanked sequences (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replace it with the recombined AttB/AttP site (AttL). Mice have both endogenous gene and Cre recombinase expression directed to Vglut3-expressing cells by the endogenous promoter/enhancer elements of the Slc17a8 locus. |
