| Primary Identifier | MGI:5804171 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Smc1b |
| Strain of Origin | FVB/NJ x B6(Cg)-Tyr<c-2J>/J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Using an sgRNA (targeting GAATATGTAGGCAAGAGTTTGAAC) and ssODN template (TTCTTAGTTTTTGAGGCCAGCAGAAAGGAAGCCAGAATATGTAGGCAAGAGTTGGAACAGGTGAAAAGACGGAGGTACGATGCTTTCAGTCAATGTTTTGAACACATCTCAGTCTCAATTGATCAA) with CRISPR/Cas9 technology, phenylalanine codon 1054 (TTT) was changed to leucine (TTG) (c.3162T>G, p.F1054L). This is the equivalent of the human p.F1055L mutation caused by SNP rs61735519. |
