| Primary Identifier | MGI:5804191 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Lancl1 |
| Strain of Origin | FVB/NHsd | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Pronuclear-stage embryos were targeted with a zinc finger nuclease pair designed to target exon 3. A founder mouse was identified carrying a 19-bp deletion at the targeted site (CTCGGGatggcactgggtacactggCTGGGCAG -> CTCGGGCTGGGCAG), which causes a reading frame shift resulting in a premature stop codon. Absence of the protein in brain, heart and liver of homozygous mutant mice was confirmed by immunoblot analysis. |
