| Primary Identifier | MGI:5804529 | Allele Type | Transgenic |
| Attribute String | Humanized sequence, Inducible, Inserted expressed sequence | Gene | Tg(tetO-TNF)15Wxlr |
| Strain of Origin | FVB/N | Induced With | doxycycline/tetracycline |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This transgene contains the tetracycline-responsive P-tight promoter (from the plasmid pTRE-Tight, BD Biosciences Clontech), which comprises a modified Tet-responsive element containing seven direct repeats of a 36-bp sequence including the 19-bp tet operator sequence followed by a minimal cytomegalovirus (CMV) promoter, driving expression of a human tumor necrosis factor alpha cDNA whose 3' untranslated region (3'-UTR) has been replaced by the SV40 polyadenylation signal. This transgenic line was estimated by Southern blot analysis to carry 5 tandem copies of the transgene. No human TNFalpha is detected by enzyme-linked immunosorbent assay (ELISA) in sera of doxycycline-treated or untreated mice bearing this transgene alone. |
