Primary Identifier | MGI:5805245 | Allele Type | Targeted |
Attribute String | Inducible, Recombinase, Reporter | Gene | Olfm4 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Induced With | tamoxifen | Is Recombinase | true |
Is Wild Type | false |
molecularNote | The targeting vector was designed to insert an eGFPCreERT2 (GCE) fusion protein coding sequence and loxp-flanked neo selection cassette into just after the stop codon of the last exon of Olfm4 gene, making use of the endogenous poly A signal. Positive clones were injected into blastocysts to establish the mouse line. The neomycin selection cassette was excised in vivo by crossing the mice with the PGK-Cre mouse strain. |