| Primary Identifier | MGI:5805836 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Mfsd2a |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 122850309 of Chromosome 4 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 122850988. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, removing the lacZ sequences and neomycin selection cassette. Exon 3 remains flanked by loxP sites. |
