| Primary Identifier | MGI:5817754 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Eps8 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 5 was flanked by loxP sites and an FRT site flanked reporter-selection double cassette was inserted into intron 4. The double cassette consists of a beta galactosidase (lacZ) gene cassette separated from a neomycin resistance gene cassette by a loxP site. The lacZ-neo double cassette was removed through flp-mediated recombination. Exon 5 was deleted by cre-mediated recombination, creating a knock-out allele. RT-PCR confirmed lack of wild-type transcripts. |
