| Primary Identifier | MGI:5825131 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Chmp7 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele from project Chmp7-8200J-F4216 was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences GGAAATGTAAAAATAACCCA, GCTGATTACTTCCGGACAGT, GTTAATATAATGCCTCTGAG and TGCGACAGACAGCCTGTCCT, which resulted in a 385 bp deletion beginning at Chromosome 14 negative strand position 69,730,348 bp CAGGCTGTCTGTCGCATTAC, and ending after TAGGGTTATATATTCCTTGG at 69,729,964 bp (GRCm38/mm10). This mutation deletes exon 2 and 213 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 100 and early truncation 25 amino acids later. |
