| Primary Identifier | MGI:5825215 | Allele Type | Transgenic |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Tg(Prnp-TARDBP*Q331K)31Dwc |
| Strain of Origin | C57BL/6 x C3H | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A full-length human TAR DNA binding protein (TARDBP or TDP-43) cDNA sequence was modified to have both an N-terminal myc tag and the glutamine to lysine substitution at amino acid 331 associated with familial ALS (huTDP-43*Q331K). This huTDP-43*Q331K cDNA sequence was inserted between exon 2 and exon 3 of mouse prion protein (Prnp) gene. The resulting Prnp-TDP-43*Q331K transgene was flanked with loxP sites. Founder line 31 has moderate overexpression levels in total TDP-43 mRNA/protein, with an ~3-fold increase in total TDP-43 expression (huTDP-43*Q331K levels ~2-fold greater) compared to endogenous TDP-43 in non-transgenic mice. |
