| First Author | Lin L | Year | 2024 |
| Journal | J Biomed Res | Pages | 1-16 |
| PubMed ID | 38807374 | Mgi Jnum | J:350433 |
| Mgi Id | MGI:7663013 | Doi | 10.7555/JBR.38.20240069 |
| Citation | Lin L, et al. (2024) DEC1 deficiency protects against bone loss induced by ovariectomy through inhibiting inflammation. J Biomed Res :1-16 |
| abstractText | Previous studies have shown that differentiated embryo-chondrocyte expressed gene 1 (DEC1) promotes osteoblast osteogenesis. To investigate the role of DEC1 in postmenopausal osteoporosis (PMOP), we utilized the two types (DEC1 (+/+), DEC1 (-/-)) mice to establish an ovariectomy (OVX) model and found that the bone loss in DEC1 (-/-) OVX mice were much less than that in DEC1 (+/+) OVX mice. The expression levels of RUNX2 and OSX significantly increased in DEC1 (-/-) OVX mice compared with those in DEC1 (+/+) OVX mice. Whereas, NFATc1, c-Fos, CTSK and RANKL/OPG significantly decreased in DEC1 (-/-) OVX mice compared with those in DEC1 (+/+) OVX mice. Likewise, DEC1 deficiency suppressed IL-6 and IL-1beta. Further study showed Runx2, Osx, Alp, and Ocn significantly increased in DEC1 (-/-) OVX BMSCs compared with those in DEC1 (+/+) OVX BMSCs. And the mRNA levels of IL-1beta, IL-6, Tnf-alpha and Ifn-gamma increased significantly in DEC1 (+/+) OVX BMMs compared with those in DEC1 (+/+) sham BMMs, but not in DEC1 (-/-) OVX BMMs compared with those in DEC1 (-/-) sham BMMs. Furthermore, the p-IkappaBalpha and p-P65 significantly increased in DEC1 (+/+) OVX BMMs compared with those in DEC1 (+/+) sham BMMs, but did not increase in DEC1 (-/-) OVX BMMs compared with those in DEC1 (-/-) sham BMMs. Taken together, DEC1 deficiency inhibits the NF-kappaB pathway induced by OVX, thereby decreasing cytokines, and subsequently, inhibits the decrease of osteogenesis and the increase of osteoclastogenesis caused by OVX. The findings provide a novel understanding of postmenopausal osteoporosis development, which offers potential avenues for the intervention strategies. |
