| First Author | Ioannidis V | Year | 2001 |
| Journal | J Immunol | Volume | 167 |
| Issue | 11 | Pages | 6256-62 |
| PubMed ID | 11714788 | Mgi Jnum | J:72831 |
| Mgi Id | MGI:2153665 | Doi | 10.4049/jimmunol.167.11.6256 |
| Citation | Ioannidis V, et al. (2001) Cre Recombinase-Mediated Inactivation of H-2D(d) Transgene Expression: Evidence for Partial Missing Self-Recognition by Ly49A NK Cells. J Immunol 167(11):6256-62 |
| abstractText | We have established H-2D(d)-transgenic (Tg) mice, in which H-2D(d) expression can be extinguished by Cre recombinase-mediated deletion of an essential portion of the transgene (Tg). NK cells adapted to the expression of the H-2D(d) Tg in H-2(b) mice and acquired reactivity to cells lacking H-2D(d), both in vivo and in vitro. H-2D(d)-Tg mice crossed to mice harboring an Mx-Cre Tg resulted in mosaic H-2D(d) expression. That abrogated NK cell reactivity to cells lacking D(d). In D(d) single Tg mice it is the Ly49A(+) NK cell subset that reacts to cells lacking D(d), because the inhibitory Ly49A receptor is no longer engaged by its D(d) ligand. In contrast, Ly49A(+) NK cells from D(d) x MxCre double Tg mice were unable to react to D(d)-negative cells. These Ly49A(+) NK cells retained reactivity to target cells that were completely devoid of MHC class I molecules, suggesting that they were not anergic. Variegated D(d) expression thus impacts specifically missing D(d) but not globally missing class I reactivity by Ly49A(+) NK cells. We propose that the absence of D(d) from some host cells results in the acquisition of only partial missing self-reactivity. |
