| Primary Identifier | MGI:5881955 | Allele Type | Transgenic |
| Attribute String | Reporter | Gene | Tg(Afp-lacZ)1Scnch |
| Strain of Origin | C57BL/6 or CBA or DBA/2 | Is Recombinase | false |
| Is Wild Type | false |
| description | "A transgenic construct...was injected into B6D2 donor eggs" (J:82809) or into "fertilized eggs from B6xCBA F1 hybrids" (J:239809). |
| molecularNote | Mice bearing this transgene express beta-galactosidase (from the E. coli lacZ gene) with a nuclear localization signal driven by a 3299-base pair (bp) fragment of the rat alpha-fetoprotein promoter, encompassing nucleotides -3217 to +102 relative to the transcription start site. This expression construct was inserted into a retroviral plasmid-derived vector in opposite orientation to a neomycin resistance gene under control of the SV40 early promoter. Beta-galactosidase is present at high levels in transgenic fetal liver, where it is restricted to hepatocytes, peaks at birth, and decreases to insignificant levels in the adult liver. |
