| Primary Identifier | MGI:5883834 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Podxl2 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 88825445 of Chromosome 6 upstream of the critical exons 3 and 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exons at position 88827396. The critical exons 3 and 4 are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exons 3 and 4. |
