| Primary Identifier | MGI:5896993 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Tm6sf2 |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 mutagenesis was used to create a mutation of a C/G base pair inserted into the coding region right after start codon, disrupting the open reading frame. The guide RNA targeted the coding region sequence 5'-GACATCCCGCCGC-3'. Western blot analysis confirmed that the expression of the encoded protein was ablated. |
