| Primary Identifier | MGI:5897899 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Cdh23 |
| Inheritance Mode | Recessive | Transmission | Germline |
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Chr10: 60,527,162 bp 60,536,889 bp (GRCm38), which contains the c.753A point mutation but is otherwise identical in sequence to the 129S1/SvImJ sequence, was taken from a C57BL/6J-derived BAC, a loxP-flanked PGK-Neo cassette was inserted 178 bp downstream of the c.753 nucleotide, homology arms were added to correctly target this sequence to replace the endogenous coding sequence, resulting in a G to A point substitution with a loxP-flanked PGK-Neo cassette. Subsequent breeding to a cre deleter strain excised the selection cassette and generated this allele. Cochlear transcripts show increased exon skipping as a result of this point mutation, and this is predicted to delete part of the 2nd and 3rd ectodomains. |
