| Primary Identifier | MGI:5898434 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Gcg |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The targeting construct is designed to insert a codon improved Cre recombinase gene, followed by an FRT-flanked neomycin resistance (neo) cassette, into exon 2 of the gene. Unexpectedly the targeting process resulted in a duplication of all exons of Gcg gene, which spans 9 kb, and extends upstream for at least 17 kb and downstream for at least 7.8 kb, including the neighboring fibroblast activation protein (Fap) gene. Flp-mediated recombination removed the FRT-flanked neo cassette. Mice express Cre in 93-95% of alpha cells and in a negligible amount of beta cells. Cre expression is also seen in intestinal L cells and some neurons in brain. |
