| Primary Identifier | MGI:5901850 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Mylk4 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele from project Mylk4-8533J-952F was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences ATATACTTCTGGTTTAACAT, AGGCTCCCTGATTTTACTGA, TTACTTGGTGCAGAAAGGAG and GCGAGAAATCTGAAGTTCCA, which resulted in a 584 bp deletion beginning at Chromosome 13 negative strand position 32,725,161 bp, GAACTTCAGATTTCTCGCTG, and ending after ATACTTCTGGTTTAACATTG at 32,724,578 bp (GRCm38/mm10). This mutation deletes exon 3 and 478 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 79 and early truncation 16 amino acids later. |
