| Primary Identifier | MGI:7780066 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | H2az2 |
| Transmission | Germline | Strain of Origin | C57BL/6NCrl |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The L1L2_Bact_P cassette was inserted, in reverse (gene) orientation, at position 6377006 of Chromosome 11 downstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 p(A) signal sequences, a second FRT site and a second loxP site. A third loxP site is inserted, in reverse (gene) orientation, into intron 3 at position 6379646. Exons 4-5 are thus flanked by loxP sites. A conditional-ready allele was created by removing the lacZ-neo cassette through subsequent Flp-mediated recombination. |
