| Primary Identifier | MGI:5903085 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Map3k14 |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site flanked neomycin resistance gene cassette was inserted into intron 1 and a further loxP site into intron 2. The neo cassette was deleted through subsequent cre-mediated recombination, leaving a conditional-ready allele with exon 2 floxed. A constitutive null allele was then created through cross-breeding with a cre allele containing strain. Absence of protein expression from the allele was confirmed by Western blots on antibody-stimulated B cells and MEFs. |
