| Primary Identifier | MGI:7860834 | Allele Type | Transgenic |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Tg(Rr27-HBB)del-2Keij |
| Strain of Origin | CD-1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The transgene insert was generated as follows: two differently modified mouse H19 ICR sequences (del-2 and del-3, each flanked by loxP5171 and loxP2272 sites) were inserted in reverse orientation in human HBB cluster-containing YAC A201F4.3 immediately downstream of the LCR and upstream of the HBB cluster. Subsequent Cre-mediated recombination created separate single del-2 and del-3 transgene alleles. The modifications involves the deletion of the 5' end of the ICR and all but the last 60 bp (del-2) or 120 (del-3) of a 478 bp sequence close to the 5' end that confers post-fertilization paternal methylation to the ICR. |
