| Primary Identifier | MGI:5903422 | Allele Type | Targeted |
| Attribute String | Hypomorph, Inducible | Gene | Sirt1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Induced With | tamoxifen | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A knock-in targeting vector was designed to fuse the region encoding the hormone-binding domain (amino acids 281-599 ) of the mouse estrogen receptor alpha to exon 9 of the C-terminal end of the SIRT1 coding region, removing all but the 3' 130 bases of the 1.8 kb 3' Sirt1 UTR. Esr1 contains a point mutation (G525R) that confers selective binding to 4-hydroxy-tamoxifen (4OHT). A loxP-flanked PGK puromycin cassette is located downstream of exon 9. Cre-mediated recombination removed the floxed puro cassette. Sirtuin activity in untreated mouse embryo fibroblasts is 30% of wild-type, increasing 130% in tamoxifen treated cells. |
