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Allele : Gt(ROSA)26Sor<tm14.1(Mir150)Rsky> gene trap ROSA 26, Philippe Soriano; targeted mutation 14.1, Klaus Rajewsky
Primary Identifier  MGI:5904751 Allele Type  Targeted
Attribute String  Conditional ready, Inserted expressed sequence Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  Not Specified
Is Recombinase  false Is Wild Type  false
description  ES cell line = R19 (ARF1 R19 ES cells from Artemis Pharmaceuticals, Germany)
molecularNote  The following elements were inserted into the locus: the CAG promoter and a loxP site flanked neomycin resistance gene and STOP cassette; genomic DNA including the microRNA stem loop with 136 bp of 5' and 130 bp of 3' flanking sequence; an frt site flanked IRES-EGFP cassette; and finally a polyadenylation signal. The EGFP cassette was deleted through subsequent FLP-mediated recombination. It is possible to generate allele Gt(ROSA)26Sortm2.1(Mirn150)Rsky from this allele.
  • mutations:
  • Insertion
  • synonyms:
  • Stop-150<fl>,
  • Rosa-26-flox-Stop-flox-miR-150<fl>,
  • Stop-150<fl>,
  • Rosa-26-flox-Stop-flox-miR-150<fl>
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1 Feature

Trail: Allele

Genome

1 Expresses

Trail: Allele

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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Trail: Allele




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