Primary Identifier | MGI:5907034 | Allele Type | Targeted |
Attribute String | Inserted expressed sequence, Null/knockout | Gene | Gata4 |
Transmission | Germline | Strain of Origin | (C57BL/6J x 129S6/SvEvTac)F1 |
Is Recombinase | false | Is Wild Type | false |
description | ES cell line = KV9, established from (C57BL/6J x 129S6/SvEvTac)F1 by Victor Lin, Columbia University. |
molecularNote | A construct containing a 3X-myc tagged Gata6 short isoform cDNA, polyA sequence and FRT flanked neo cassette replaced exon 2 downstream of the start signal. Flp mediated recombination removed the neo cassette. Western blot analysis confirmed the expression of the myc-tagged GATA6. |