| Primary Identifier | MGI:5905043 | Allele Type | Targeted |
| Attribute String | Epitope tag, Hypomorph | Gene | Six3 |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site flanked neomycin resistance gene cassette was inserted upstream of exon 1. A 15 amino-acid Avi tag was inserted in-frame with the gene so that the resulting translated peptide would have an N-terminal biotinylation tag. The insertion of the neo cassette is intended to interfere with transcription of the gene and was shown by RT-qPCR to indeed reduce expression to around 27% of wild-type. |
