| Primary Identifier | MGI:5908448 | Allele Type | Targeted |
| Gene | Sirt1 | Transmission | Germline |
| Strain of Origin | C57BL/6NTac | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | An A>G point mutation was engineered to change codon 522 in exon 8 from threonine to alanine (p.Thre522Ala). Alanine is non-phosphorylatable, rendering the protein constitutively inactive. A loxP site and an FRT site flanked selectable marker cassette were inserted into intron 7. The marker cassette was removed through subsequent flp-mediated recombination. |
