| Primary Identifier | MGI:5908449 | Allele Type | Targeted |
| Gene | Sirt1 | Transmission | Germline |
| Strain of Origin | C57BL/6NTac | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Two substitutions (AC>GA) were engineered to change codon 522 in exon 8 from threonine to glutamine (p.Thre522Glu). Glutamine is a phosphomimetic, simulating a phosphorylated threonine and rendering the protein constitutively active. A loxP site and an FRT site flanked selectable marker cassette were inserted into intron 7. The marker cassette was removed through subsequent flp-mediated recombination. |
