| Primary Identifier | MGI:5904780 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Tmem171 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele from project Tmem171-8631J-5335M was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences CATCAAAGCTCACAGAGCAG, GCTGAGTGTGAGCAGCGAGG, GAAACACCCTTGAGTGCGCG and ACTTGACATGGAACTGTCGC, which resulted in a 359 bp deletion beginning at Chromosome 13 negative strand position 98,688,580 bp, ACTCAAGGGTGTTTCGGCCT, and ending after TGTTTGAGATCCCGCCTCGC at 98,688,222 bp (GRCm38/mm10). This mutation deletes exon 3 and 223 bp of flanking intronic sequence including the splice acceptor and donor. In addition there is a 6 bp (CTGTCG) intronic deletion 25 bp before the 359 bp deletion that will not alter the results of the exon deletion. This mutation is predicted to cause a change of amino acid sequence after residue 213 and early truncation 86 amino acids later. |
