| Primary Identifier | MGI:5911848 | Allele Type | Endonuclease-mediated |
| Attribute String | Hypomorph | Gene | Rr492 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas technology was employed to introduce loxP sequences flanking a 13-kb enhancer residing 132 kb upstream of the promoter (Pitx distal enhancer or PDE), shown via chromatin conformation capture (3C) and chromosome conformation capture-on-chip (4C) analyses to interact topologically with the target gene in hind limbs The floxed sequence was deleted by Cre recombinase. The deleted sequence spans chr13:56102380-56115399 (GRCm39). In heterozygous mutant embryos at E11.5, Pitx1 expression is reduced by 15% in the hindlimb and by 39% in the mandibular arch. |
