| Primary Identifier | MGI:5905785 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Trp53i11 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele from project Trp53i11-8646J-9616F was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences GATGGCCCCCAGGTGTCCAC, GAGAGGTTGAGCTTGCATTG, GTAGGACCTTACACATACCC and CTGTGCAACCCAAACAGCCA, which resulted in a 235 bp deletion beginning at Chromosome 2 positive strand position 93,198,127 bp, TTGGGGAAAACATTTATGTT, and ending after GGATGGTTTTGTGTCCCGGG at 93,198,361 bp (GRCm38/mm10). This mutation deletes exon 4 and 176 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 43 and early truncation 21 amino acids later. |
