| Primary Identifier | MGI:5907280 | Allele Type | Targeted |
| Gene | Gucy1b1 | Transmission | Germline |
| Strain of Origin | 129S/SvEv | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Exon 5 was replaced with a mutant version carrying an H105F point mutation and a floxed pgk-neo cassette was inserted downstream of exon 5 via homologous recombination. Cre-mediated recombination removed the neo cassette. The replacement of the wild-type histidine with a phenylalanine interferes with haem ligation resulting in loss of NO activation. |
