| First Author | Reimann F | Year | 2008 |
| Journal | Cell Metab | Volume | 8 |
| Issue | 6 | Pages | 532-9 |
| PubMed ID | 19041768 | Mgi Jnum | J:238690 |
| Mgi Id | MGI:5823355 | Doi | 10.1016/j.cmet.2008.11.002 |
| Citation | Reimann F, et al. (2008) Glucose sensing in L cells: a primary cell study. Cell Metab 8(6):532-9 |
| abstractText | Glucagon-like peptide-1 (GLP-1) is an enteric hormone that stimulates insulin secretion and improves glycaemia in type 2 diabetes. Although GLP-1-based treatments are clinically available, alternative strategies to increase endogenous GLP-1 release from L cells are hampered by our limited physiological understanding of this cell type. By generating transgenic mice with L cell-specific expression of a fluorescent protein, we studied the characteristics of primary L cells by electrophysiology, fluorescence calcium imaging, and expression analysis and show that single L cells are electrically excitable and glucose responsive. Sensitivity to tolbutamide and low-millimolar concentrations of glucose and alpha-methylglucopyranoside, assessed in single L cells and by hormone secretion from primary cultures, suggested that GLP-1 release is regulated by the activity of sodium glucose cotransporter 1 and ATP-sensitive K(+) channels, consistent with their high expression levels in purified L cells by quantitative RT-PCR. These and other pathways identified using this approach will provide exciting opportunities for future physiological and therapeutic exploration. |
