Primary Identifier | MGI:5924942 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Adgrf5 |
Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | DNA sequence comprising the beta-galactosidase (lacZ) reporter gene and a loxP site flanked neomycin resistance gene driven by human UBC and bacterial EM7 promoters, replaced a 32 kbp region of the target gene spanning exons 4 to 21. Severely reduced transcription from this knock-out allele was shown by qRT-PCR. No lacZ activity was detected by either qRT-PCR or enzymatic assays. |