| First Author | Roth S | Year | 2009 |
| Journal | Genesis | Volume | 47 |
| Issue | 1 | Pages | 7-13 |
| PubMed ID | 18942097 | Mgi Jnum | J:144641 |
| Mgi Id | MGI:3831460 | Doi | 10.1002/dvg.20446 |
| Citation | Roth S, et al. (2009) Generation of a tightly regulated doxycycline-inducible model for studying mouse intestinal biology. Genesis 47(1):7-13 |
| abstractText | To develop a sensitive and inducible system to study intestinal biology, we generated a transgenic mouse model expressing the reverse tetracycline transactivator rtTA2-M2 under control of the 12.4 kb murine Villin promoter. The newly generated Villin-rtTA2-M2 mice were then bred with the previously developed tetO-HIST1H2BJ/GFP model to assess inducibility and tissue-specificity. Expression of the histone H2B-GFP fusion protein was observed exclusively upon doxycycline induction and was uniformly distributed throughout the intestinal epithelium. The Villin-rtTA2-M2 was also found to drive transgene expression in the developing mouse intestine. Furthermore, we could detect transgene expression in the proximal tubules of the kidney and in a population of alleged gastric progenitor cells. By administering different concentrations of doxycycline, we show that the Villin-rtTA2-M2 system drives transgene expression in a dosage-dependent fashion. Thus, we have generated a novel doxycycline-inducible mouse model, providing a valuable tool to study the effect of different gene dosages on intestinal physiology and pathology. |
