Primary Identifier | MGI:6158939 | Allele Type | Transgenic |
Attribute String | Inserted expressed sequence, Reporter | Gene | Tg(Nkx2-2-EGFP)2Mtse |
Strain of Origin | C57BL/6 or SJL | Is Recombinase | false |
Is Wild Type | false |
description | "BAC DNA was microinjected into the male pronucleus of fertilized oocytes from C57BL/6 mice crossed with SJL agouti mice." (J: J:214964) |
molecularNote | The bacterial artificial chromosome (BAC) RP23-224O8, modified by GENSAT to insert the coding sequence for enhanced green fluorescent protein (EGFP) downstream of the 5' regulatory sequences of the mouse NK2 homeobox 2 gene (Nkx2.2), was obtained from CHORI and microinjected into the male pronuclei of fertilized oocytes. Founder line 2 exhibited the strongest EGFP expression. |