| First Author | Yu X | Year | 2017 |
| Journal | Nat Commun | Volume | 8 |
| Pages | 14016 | PubMed ID | 28091529 |
| Mgi Jnum | J:243999 | Mgi Id | MGI:5912778 |
| Doi | 10.1038/ncomms14016 | Citation | Yu X, et al. (2017) Long non-coding RNA Linc-RAM enhances myogenic differentiation by interacting with MyoD. Nat Commun 8:14016 |
| abstractText | Long non-coding RNAs (lncRNAs) are important regulators of diverse biological processes. Here we report on functional identification and characterization of a novel long intergenic non-coding RNA with MyoD-regulated and skeletal muscle-restricted expression that promotes the activation of the myogenic program, and is therefore termed Linc-RAM (Linc-RNA Activator of Myogenesis). Linc-RAM is transcribed from an intergenic region of myogenic cells and its expression is upregulated during myogenesis. Notably, in vivo functional studies show that Linc-RAM knockout mice display impaired muscle regeneration due to the differentiation defect of satellite cells. Mechanistically, Linc-RAM regulates expression of myogenic genes by directly binding MyoD, which in turn promotes the assembly of the MyoD-Baf60c-Brg1 complex on the regulatory elements of target genes. Collectively, our findings reveal the functional role and molecular mechanism of a lineage-specific Linc-RAM as a regulatory lncRNA required for tissues-specific chromatin remodelling and gene expression. |
