| Primary Identifier | MGI:6108137 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Otud7a |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Two single guide RNA (sgRNA) sequences were designed to delete a portion of exon 4, all of exon 5 and a portion of exon 6 of the gene. Deletion of that region (encoding most of the OTU deubiquitinase domain) creates a premature stop codon in exon 8. No off-target mutations were found (via sequencing the top fifteen off-target candidates for each sgRNA predicted by CRISPR design tool). |
