| Primary Identifier | MGI:6107687 | Allele Type | Targeted |
| Attribute String | Humanized sequence | Gene | Thap1 |
| Transmission | Germline | Strain of Origin | 129S/SvEv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted into intron 1 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 2. The neo cassette was removed through subsequent flp-mediated recombination, leaving exon 2 floxed. A G>A substitution was engineered in exon 2, changing cysteine codon 54 to a tyrosine codon (p.C54Y or p.Cys54Tyr). This mutation in the DNA-binding domain mimics mutations found in human torsion dystonia 6 patients. |
